Title
Linking SNPs to CAG repeat length in Huntington's disease patients
PubMed ID
18931668
UMMS Affiliation
Department of Medicine, Division of Endocrinology and Metabolism; Department of Biochemistry and Molecular Pharmacology
Date
10-22-2008
Document Type
Article
Subjects
Humans; Huntington Disease; Molecular Sequence Data; Polymorphism, Single Nucleotide; Trinucleotide Repeats
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
Allele-specific silencing using small interfering RNAs targeting heterozygous single-nucleotide polymorphisms (SNPs) is a promising therapy for human trinucleotide repeat diseases such as Huntington's disease. Linking SNP identities to the two HTT alleles, normal and disease-causing, is a prerequisite for allele-specific RNA interference. Here we describe a method, SNP linkage by circularization (SLiC), to identify linkage between CAG repeat length and nucleotide identity of heterozygous SNPs using Huntington's disease patient peripheral blood samples.
Rights and Permissions
Citation: Nat Methods. 2008 Nov;5(11):951-3. Epub 2008 Oct 19. Link to article on publisher's site