Title

Radiolabeling of MAG3-morpholino oligomers with 188Re at high labeling efficiency and specific radioactivity for tumor pretargeting

UMMS Affiliation

Division of Nuclear Medicine; Department of Radiology

Date

5-30-2006

Document Type

Article

Subjects

Animals; Chromatography, High Pressure Liquid; Isotope Labeling; Mice; Morpholines; Oligopeptides; Organometallic Compounds; Radioisotopes; Radiopharmaceuticals; Rhenium

Disciplines

Life Sciences | Medicine and Health Sciences | Radiology

Abstract

We are investigating a novel pretargeting approach involving an initial IV injection of antitumor antibody conjugated with a phosphorodiamidate morpholino oligomer (MORF, a DNA analog) and the subsequent IV injection of the radiolabeled complement oligomer (cMORF). In this paper, the cMORF was labeled with (188)Re using MAG(3) as chelator for therapeutic applications. Since (c)MORFs are unstable in acidic condition, an optimal labeling pH was first selected and the other labeling factors were then examined. A labeling efficiency of greater than 90% can be achieved even at a concentration of MAG(3)-cMORF as low as 0.8 microM. The labeled cMORF is stable and capable of hybridizing to its complement.

Rights and Permissions

Citation: Appl Radiat Isot. 2006 Sep;64(9):971-8. Epub 2006 May 30. Link to article on publisher's site

DOI of Published Version

10.1016/j.apradiso.2006.04.005

Related Resources

Link to article in PubMed

Journal Title

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine

PubMed ID

16730997