RNA target specificity of the embryonic cell fate determinant POS-1
Department of Biochemistry and Molecular Pharmacology
Animals; Base Sequence; Binding Sites; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Carrier Proteins; Consensus Sequence; Embryonic Development; RNA, Messenger; RNA-Binding Proteins; Untranslated Regions
Life Sciences | Medicine and Health Sciences
Specification of Caenorhabditis elegans body axes and cell fates occurs prior to the activation of zygotic transcription. Several CCCH-type tandem zinc finger (TZF) proteins coordinate local activation of quiescent maternal mRNAs after fertilization, leading to asymmetric expression of factors required for patterning. The primary determinant of posterior fate is the TZF protein POS-1. Mutants of pos-1 are maternal effect lethal with a terminal phenotype that includes excess pharyngeal tissue and no endoderm or germline. Here, we delineate the consensus POS-1 recognition element (PRE) required for specific recognition of its target mRNAs. The PRE is necessary but not sufficient to pattern the expression of a reporter. The PRE is distinct from sequences recognized by related proteins from both mammals and nematodes, demonstrating that variants of this protein family can recognize divergent RNA sequences. The PRE is found within the 3' untranslated region of 227 maternal transcripts required for early development, including genes involved in endoderm and germline specification. The results enable prediction of novel targets that explain the pleiotropy of the pos-1 phenotype.
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Citation: RNA. 2008 Dec;14(12):2685-97. Epub 2008 Oct 24. Link to article on publisher's site
Farley, Brian M.; Pagano, John M.; and Ryder, Sean P., "RNA target specificity of the embryonic cell fate determinant POS-1" (2008). Open Access Articles. Paper 1929.