PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C. elegans
Program in Molecular Medicine
Animals; Base Sequence; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Fertility; Gene Expression Regulation; Germ Cells; Models, Biological; Molecular Sequence Data; Mutation; Protein Binding; RNA, Helminth; RNA, Small Interfering; RNA-Induced Silencing Complex; Regulatory Sequences, Nucleic Acid
Life Sciences | Medicine and Health Sciences
In metazoans, Piwi-related Argonaute proteins have been linked to germline maintenance, and to a class of germline-enriched small RNAs termed piRNAs. Here we show that an abundant class of 21 nucleotide small RNAs (21U-RNAs) are expressed in the C. elegans germline, interact with the C. elegans Piwi family member PRG-1, and depend on PRG-1 activity for their accumulation. The PRG-1 protein is expressed throughout development and localizes to nuage-like structures called P granules. Although 21U-RNA loci share a conserved upstream sequence motif, the mature 21U-RNAs are not conserved and, with few exceptions, fail to exhibit complementarity or evidence for direct regulation of other expressed sequences. Our findings demonstrate that 21U-RNAs are the piRNAs of C. elegans and link this class of small RNAs and their associated Piwi Argonaute to the maintenance of temperature-dependent fertility.
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Citation: Mol Cell. 2008 Jul 11;31(1):67-78. Epub 2008 Jun 19. Link to article on publisher's site
DOI of Published Version
Batista, Pedro J.; Ruby, J. Graham; Claycomb, Julie M.; Chiang, H. Rosaria; Fahlgren, Noah; Kasschau, Kristin D.; Chaves, Daniel A.; Gu, Weifeng; Vasale, Jessica J.; Duan, Shenghua; Conte, Darryl; Luo, Shujun; Schroth, Gary P.; Carrington, James C.; Bartel, David P.; and Mello, Craig C., "PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C. elegans" (2008). Open Access Articles. 1908.