Title

Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement

UMMS Affiliation

Program in Molecular Medicine

Publication Date

4-12-1994

Document Type

Article

Subjects

Adenosine Triphosphate; Chromatography, Affinity; Chromosomal Proteins, Non-Histone; DNA-Binding Proteins; Deoxyribonucleoproteins; Fungal Proteins; Macromolecular Substances; Nuclear Proteins; Protein Binding; Saccharomyces cerevisiae; *Saccharomyces cerevisiae Proteins; *Trans-Activators; Transcription Factors; *Transcription, Genetic

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The Saccharomyces cerevisiae SWI1, SWI2 (SNF2), SWI3, SNF5, and SNF6 gene products play a crucial role in the regulation of transcription. We provide here direct biochemical evidence that all five SWI/SNF polypeptides are components of a large multisubunit complex. These five polypeptides coelute from a gel-filtration column with an apparent molecular mass of approximately 2 MDa. The five SWI/SNF polypeptides do not copurify when extracts are prepared from swi- or snf- mutants. We show that SWI/SNF polypeptides also remain associated during an affinity-chromatography step followed by gel filtration. Assembly of the SWI/SNF complex is not disrupted by a mutation in the putative APT-binding site of SWI2, although this mutation eliminates SWI2 function.

Rights and Permissions

Citation: Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):2905-8.

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Proceedings of the National Academy of Sciences of the United States of America

PubMed ID

8159677