Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement
Program in Molecular Medicine
Adenosine Triphosphate; Chromatography, Affinity; Chromosomal Proteins, Non-Histone; DNA-Binding Proteins; Deoxyribonucleoproteins; Fungal Proteins; Macromolecular Substances; Nuclear Proteins; Protein Binding; Saccharomyces cerevisiae; *Saccharomyces cerevisiae Proteins; *Trans-Activators; Transcription Factors; *Transcription, Genetic
Life Sciences | Medicine and Health Sciences
The Saccharomyces cerevisiae SWI1, SWI2 (SNF2), SWI3, SNF5, and SNF6 gene products play a crucial role in the regulation of transcription. We provide here direct biochemical evidence that all five SWI/SNF polypeptides are components of a large multisubunit complex. These five polypeptides coelute from a gel-filtration column with an apparent molecular mass of approximately 2 MDa. The five SWI/SNF polypeptides do not copurify when extracts are prepared from swi- or snf- mutants. We show that SWI/SNF polypeptides also remain associated during an affinity-chromatography step followed by gel filtration. Assembly of the SWI/SNF complex is not disrupted by a mutation in the putative APT-binding site of SWI2, although this mutation eliminates SWI2 function.
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Citation: Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):2905-8.
Proceedings of the National Academy of Sciences of the United States of America
Peterson, Craig L.; Dingwall, Andrew K.; and Scott, Matthew P., "Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement" (1994). Open Access Articles. 1809.