Title

Mitogen-activated protein kinase kinase 7 is an activator of the c-Jun NH2-terminal kinase

UMMS Affiliation

Program in Molecular Medicine; Howard Hughes Medical Institute

Publication Date

7-8-1997

Document Type

Article

Subjects

Amino Acid Sequence; Animals; CHO Cells; Cloning, Molecular; Cricetinae; *Gene Expression Regulation, Enzymologic; Humans; *JNK Mitogen-Activated Protein Kinases; MAP Kinase Kinase 4; MAP Kinase Kinase 7; Mitogen-Activated Protein Kinase Kinases; Molecular Sequence Data; Protein Kinases; Sequence Alignment

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The c-Jun NH2-terminal kinase (JNK) group of mitogen-activated protein (MAP) kinases is activated by phosphorylation on Thr and Tyr. Here we report the molecular cloning of a new member of the mammalian MAP kinase kinase group (MKK7) that functions as an activator of JNK. In vitro protein kinase assays demonstrate that MKK7 phosphorylates and activates JNK, but not the p38 or extracellular signal-regulated kinase groups of MAP kinase. Expression of MKK7 in cultured cells causes activation of the JNK signal transduction pathway. MKK7 is therefore established to be a novel component of the JNK signal transduction pathway.

Rights and Permissions

Citation: Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7337-42.

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Proceedings of the National Academy of Sciences of the United States of America

PubMed ID

9207092