Title

An ectopic human XIST gene can induce chromosome inactivation in postdifferentiation human HT-1080 cells

UMMS Affiliation

Department of Cell Biology

Date

6-20-2002

Document Type

Article

Subjects

Acetylation; Cell Differentiation; *Chromosomes, Human; Gene Silencing; Humans; In Situ Hybridization, Fluorescence; Male; Microscopy, Fluorescence; RNA; RNA, Untranslated; Transcription Factors; Transcription, Genetic; Tumor Cells, Cultured

Disciplines

Cell Biology | Life Sciences | Medicine and Health Sciences

Abstract

It has been believed that XIST RNA requires a discrete window in early development to initiate the series of chromatin-remodeling events that form the heterochromatic inactive X chromosome. Here we investigate four adult male HT-1080 fibrosarcoma cell lines expressing ectopic human XIST and demonstrate that these postdifferentiation cells can undergo chromosomal inactivation outside of any normal developmental context. All four clonal lines inactivated the transgene-containing autosome to varying degrees and with variable stability. One clone in particular consistently localized the ectopic XIST RNA to a discrete chromosome territory that exhibited striking hallmarks of inactivation, including long-range transcriptional inactivation. Results suggest that some postdifferentiation cell lines are capable of de novo chromosomal inactivation; however, long-term retention of autosomal inactivation was less common, which suggests that autosomal inactivation may confer a selective disadvantage. These results have fundamental significance for understanding genomic programming in early development.

Rights and Permissions

Citation: Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8677-82. Epub 2002 Jun 18. Link to article on publisher's site

Related Resources

Link to Article in PubMed

PubMed ID

12072569