UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology

Publication Date

10-25-1988

Document Type

Article

Subjects

Base Composition; Base Sequence; *Chromosomes; Codon; DNA, Bacterial; Escherichia coli; *Genes, Bacterial; Genetic Code; Methylation; Nucleic Acid Conformation; Oligonucleotides; Operon

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The occurrence of GATC (Dam-recognition) sites in available E. coli DNA sequences (representing about 2% of the chromosome) has been determined by a simple numerical analysis. Our approach was to analyze the nucleotide composition of nine large sequenced DNA stretches ("cantles") in order to identify patterns of GATC distribution and to rationalize such patterns in biological/structural terms. The following observations were made: (i) In addition to oriC, GATC-rich regions are present in numerous locations. (ii) There is a wide variation in GATC frequency both between and within DNA cantles which led to the identification of a void-cluster pattern of GATC arrangement. The distance between two GATCs was never greater than 2 kb. (iii) GATC sites are found more frequently in translated regions than (in decreasing order) non-coding or non-translated regions. In particular, rRNA and tRNA encoding genes exhibit the lowest GATC content.

Rights and Permissions

Citation: Nucleic Acids Res. 1988 Oct 25;16(20):9821-38.

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Nucleic acids research

PubMed ID

3054812

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.