UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology

Date

4-11-1991

Document Type

Article

Subjects

Adenosine Triphosphate; Animals; Base Sequence; Cattle; DNA-Directed DNA Polymerase; Deoxyguanine Nucleotides; Kinetics; Molecular Sequence Data; *Polymers; T-Phages; Templates, Genetic; Thymus Gland

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Calf thymus DNA polymerase alpha (pol alpha) and bacteriophage T4 DNA polymerase (pol T4) were exploited as model enzymes to investigate the molecular mechanism of inhibitory action of N2-(p-n-butylphenyl)dGTP (BuPdGTP) and 2-(p-n-butyl-anilino)dATP (BuAdATP) on the BuPdNTP-susceptible alpha polymerase family. Kinetic analysis of inhibition of pol alpha with mixtures of complementary and noncomplementary template:primers indicated that both nucleotides induced the formation of a polymerase: inhibitor:primer-template complex. Primer extension experiments using the guanine form as the model analog indicated that pol alpha cannot utilize these nucleotides to extend primer termini. In contrast, pol T4 polymerized BuPdGTP, indicating that resistance to polymerization is not a common feature of the inhibitor mechanism among the broad membership of the alpha polymerase family.

Rights and Permissions

Citation: Nucleic Acids Res. 1991 Apr 11;19(7):1627-32.

Related Resources

Link to Article in PubMed

Journal Title

Nucleic acids research

PubMed ID

2027770

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