Program in Molecular Medicine
Activating Transcription Factors; Animals; Binding Sites; Blood Proteins; Cell Line; Cyclic AMP Response Element-Binding; Protein; DNA; Fungal Proteins; Gene Expression; Haplorhini; Macromolecular Substances; Plasmids; Protein Kinases; Recombinant Fusion Proteins; *Saccharomyces cerevisiae Proteins; Transcription Factors; Transcription, Genetic
Life Sciences | Medicine and Health Sciences
Cyclic AMP response element binding protein (CREB) activates transcription of cAMP response element (CRE)-containing promoters following an elevation of intracellular cAMP. Here we show that CREB and the highly related protein ATF-1 are also potent transcription inhibitors. Strikingly, CREB inhibits transcription of multiple activators, whose DNA-binding domains and activation regions are unrelated to one another. Inhibition requires that the CREB dimerization and DNA-binding domains are intact. However, inhibition is not dependent upon the presence of a CRE in the promoter, and does not involve heterodimer formation between CREB and the activator. The ability of an activator protein to inhibit transcription in such a promiscuous fashion has not been previously reported.
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Citation: Nucleic Acids Res. 1993 Jun 25;21(12):2907-11.
Nucleic acids research
Lemaigre, Frederic P.; Ace, Christopher I.; and Green, Michael R., "The cAMP response element binding protein, CREB, is a potent inhibitor of diverse transcriptional activators" (1993). Open Access Articles. 1708.