UMMS Affiliation

Program in Molecular Medicine

Publication Date

6-25-1993

Document Type

Article

Subjects

Activating Transcription Factors; Animals; Binding Sites; Blood Proteins; Cell Line; Cyclic AMP Response Element-Binding; Protein; DNA; Fungal Proteins; Gene Expression; Haplorhini; Macromolecular Substances; Plasmids; Protein Kinases; Recombinant Fusion Proteins; *Saccharomyces cerevisiae Proteins; Transcription Factors; Transcription, Genetic

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Cyclic AMP response element binding protein (CREB) activates transcription of cAMP response element (CRE)-containing promoters following an elevation of intracellular cAMP. Here we show that CREB and the highly related protein ATF-1 are also potent transcription inhibitors. Strikingly, CREB inhibits transcription of multiple activators, whose DNA-binding domains and activation regions are unrelated to one another. Inhibition requires that the CREB dimerization and DNA-binding domains are intact. However, inhibition is not dependent upon the presence of a CRE in the promoter, and does not involve heterodimer formation between CREB and the activator. The ability of an activator protein to inhibit transcription in such a promiscuous fashion has not been previously reported.

Rights and Permissions

Citation: Nucleic Acids Res. 1993 Jun 25;21(12):2907-11.

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Nucleic acids research

PubMed ID

8332500

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