UMMS Affiliation

Department of Biochemistry and Molecular Biology

Date

2-15-1996

Document Type

Article

Subjects

Base Composition; Base Sequence; DNA Transposable Elements; DNA, Fungal; DNA, Single-Stranded; DNA-Binding Proteins; *Fungal Proteins; Molecular Sequence Data; MutS Homolog 2 Protein; Oligonucleotides; Saccharomyces cerevisiae; *Saccharomyces cerevisiae Proteins; Sequence Deletion

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

An activity in nuclear extracts of S.cerevisiae binds specifically to heteroduplexes containing four to nine extra bases in one strand. The specificity of this activity (IMR, for insertion mismatch recognition) in band shift assays was confirmed by competition experiments. IMR is biochemically and genetically distinct from the MSH2 dependent, single base mismatch binding activity. The two activities migrate differently during electrophoresis, they are differentially competable and their spectra of mispair binding are distinct. Furthermore, IMR activity is observed in extracts from an msh2- msh3- msh4- strain. IMR exhibits specificity for insertion mispairs in two different sequence contexts. Binding is influenced by the structure of the mismatch since an insertion with a hairpin configuration is not recognized by this activity. IMR does not result from single-strand binding because single-stranded probes to not yield IMR complex and single-stranded competitors are unable to displace insertion heteroduplexes from the complex. Similar results with intrinsically bent duplexes make it unlikely that recognition is conferred by a bend alone. Heteroduplexes bound by IMR do not contain any obvious damage. These findings are consistent with the idea that yeast contains a distinct recognition factor, IMR that is specific for insertion/deletion mismatches.

Rights and Permissions

Citation: Nucleic Acids Res. 1996 Feb 15;24(4):721-9.

Related Resources

Link to Article in PubMed

Journal Title

Nucleic acids research

PubMed ID

8604316

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