A bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors
UMass Chan Affiliations
Program in Gene Function and ExpressionDocument Type
Journal ArticlePublication Date
2005-07-26Keywords
AnimalsBinding Sites
DNA, Bacterial
Drosophila Proteins
Drosophila melanogaster
Escherichia coli
Genes, Regulator
Protein Binding
Protein Interaction Mapping
Sensitivity and Specificity
Sequence Analysis
Transcription Factors
*Two-Hybrid System Techniques
Life Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
The DNA-binding specificities of transcription factors can be used to computationally predict cis-regulatory modules (CRMs) that regulate gene expression. However, the absence of specificity data for the majority of transcription factors limits the widespread implementation of this approach. We have developed a bacterial one-hybrid system that provides a simple and rapid method to determine the DNA-binding specificity of a transcription factor. Using this technology, we successfully determined the DNA-binding specificity of seven previously characterized transcription factors and one novel transcription factor, the Drosophila melanogaster factor Odd-skipped. Regulatory targets of Odd-skipped were successfully predicted using this information, demonstrating that the data produced by the bacterial one-hybrid system are relevant to in vivo function.Source
Nat Biotechnol. 2005 Aug;23(8):988-94. Epub 2005 Jul 24. Link to article on publisher's site
DOI
10.1038/nbt1120Permanent Link to this Item
http://hdl.handle.net/20.500.14038/38847PubMed ID
16041365Related Resources
ae974a485f413a2113503eed53cd6c53
10.1038/nbt1120