Title

Controlling gene expression in living cells through small molecule-RNA interactions

UMMS Affiliation

Program in Molecular Medicine

Publication Date

10-9-1998

Document Type

Article

Subjects

Animals; Anti-Bacterial Agents; Base Sequence; Benzimidazoles; Bisbenzimide; CHO Cells; Cricetinae; Drug Resistance, Microbial; Escherichia coli; *Gene Expression Regulation; Kanamycin; Ligands; Molecular Sequence Data; Protein Biosynthesis; RNA; RNA, Messenger; Tobramycin; Transfection

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Short RNA aptamers that specifically bind to a wide variety of ligands in vitro can be isolated from randomized pools of RNA. Here it is shown that small molecule aptamers also bound their ligand in vivo, enabling development of a method for controlling gene expression in living cells. Insertion of a small molecule aptamer into the 5' untranslated region of a messenger RNA allowed its translation to be repressible by ligand addition in vitro as well as in mammalian cells. The ability of small molecules to control expression of specific genes could facilitate studies in many areas of biology and medicine.

Rights and Permissions

Citation: Science. 1998 Oct 9;282(5387):296-8.

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Science (New York, N.Y.)

PubMed ID

9765156