Title

Requirement of Cdk2-cyclin E activity for repeated centrosome reproduction in Xenopus egg extracts

UMMS Affiliation

Department of Cell Biology

Date

2-5-1999

Document Type

Article

Subjects

Animals; Aphidicolin; Blastomeres; *CDC2-CDC28 Kinases; *Cell Cycle Proteins; Cell Extracts; Centrosome; Cyclin E; Cyclin-Dependent Kinase 2; Cyclin-Dependent Kinase Inhibitor p27; Cyclin-Dependent Kinases; DNA; Enzyme Inhibitors; Microscopy, Confocal; Microscopy, Fluorescence; Microscopy, Video; Microtubule-Associated Proteins; Ovum; Protein Kinases; Protein-Serine-Threonine Kinases; Proteins; Recombinant Proteins; *S Phase; *Tumor Suppressor Proteins; Xenopus; Xenopus Proteins

Disciplines

Cell Biology | Life Sciences | Medicine and Health Sciences

Abstract

The abnormally high number of centrosomes found in many human tumor cells can lead directly to aneuploidy and genomic instability through the formation of multipolar mitotic spindles. To facilitate investigation of the mechanisms that control centrosome reproduction, a frog egg extract arrested in S phase of the cell cycle that supported repeated assembly of daughter centrosomes was developed. Multiple rounds of centrosome reproduction were blocked by selective inactivation of cyclin-dependent kinase 2-cyclin E (Cdk2-E) and were restored by addition of purified Cdk2-E. Confocal immunomicroscopy revealed that cyclin E was localized at the centrosome. These results demonstrate that Cdk2-E activity is required for centrosome duplication during S phase and suggest a mechanism that could coordinate centrosome reproduction with cycles of DNA synthesis and mitosis.

Rights and Permissions

Citation: Science. 1999 Feb 5;283(5403):851-4.

Related Resources

Link to Article in PubMed

Journal Title

Science (New York, N.Y.)

PubMed ID

9933170