Requirement of a centrosomal activity for cell cycle progression through G1 into S phase
Department of Cell Biology
Animals; Antigens; Cell Division; Cell Line; Centrioles; Centrosome; Cercopithecus aethiops; Cytoplasmic Granules; *G1 Phase; Interphase; Microscopy, Video; Microtubule-Organizing Center; Microtubules; Mitosis; Mitotic Spindle Apparatus; Paclitaxel; *S Phase; Tubulin
Cell Biology | Life Sciences | Medicine and Health Sciences
Centrosomes were microsurgically removed from BSC-1 African green monkey kidney cells before the completion of S phase. Karyoplasts (acentrosomal cells) entered and completed mitosis. However, postmitotic karyoplasts arrested before S phase, whereas adjacent control cells divided repeatedly. Postmitotic karyoplasts assembled a microtubule-organizing center containing gamma-tubulin and pericentrin, but did not regenerate centrioles. These observations reveal the existence of an activity associated with core centrosomal structures-distinct from elements of the microtubule-organizing center-that is required for the somatic cell cycle to progress through G1 into S phase. Once the cell is in S phase, these core structures are not needed for the G2-M phase transition.
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Citation: Science. 2001 Feb 23;291(5508):1547-50. Link to article on publisher's site
Hinchcliffe, Edward H.; Miller, Frederick J.; Cham, Matthew; Khodjakov, Alexey L.; and Sluder, Greenfield, "Requirement of a centrosomal activity for cell cycle progression through G1 into S phase" (2001). Open Access Articles. Paper 1638.