Title

A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology

Date

7-14-2001

Document Type

Article

Subjects

Animals; Blotting, Northern; Drosophila melanogaster; Endoribonucleases; *Gene Expression Regulation, Developmental; Hela Cells; Humans; Nucleic Acid Conformation; Protein Structure, Tertiary; RNA Precursors; RNA Processing, Post-Transcriptional; RNA, Double-Stranded; RNA, Helminth; RNA, Messenger; Ribonuclease III; Transcription, Genetic; Transfection

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The 21-nucleotide small temporal RNA (stRNA) let-7 regulates developmental timing in Caenorhabditis elegans and probably in other bilateral animals. We present in vivo and in vitro evidence that in Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 stRNA. Targeted destruction in cultured human cells of the messenger RNA encoding the enzyme Dicer, which acts in the RNA interference pathway, leads to accumulation of the let-7 precursor. Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression.

Rights and Permissions

Citation: Science. 2001 Aug 3;293(5531):834-8. Epub 2001 Jul 12. Link to article on publisher's site

DOI of Published Version

10.1126/science.1062961

Related Resources

Link to Article in PubMed

Journal Title

Science (New York, N.Y.)

PubMed ID

11452083