PubMed ID

11425161

UMMS Affiliation

Center for Infectious Disease and Vaccine Research; Department of Medicine, Division of Infectious Diseases and Immunology

Date

6-27-2001

Document Type

Article

Subjects

Adolescent; Child; Child, Preschool; Dengue; Dengue Hemorrhagic Fever; Female; Genes, T-Cell Receptor beta; Humans; Infant; Male; Reverse Transcriptase Polymerase Chain Reaction; Severity of Illness Index; Thailand

Disciplines

Immunology and Infectious Disease | Pediatrics

Abstract

T lymphocyte activation during dengue is thought to contribute to the pathogenesis of dengue hemorrhagic fever (DHF). We examined the T cell receptor Vbeta gene usage by a reverse transcriptase-polymerase chain reaction assay during infection and after recovery in 13 children with DHF and 13 children with dengue fever (DF). There was no deletion of specific Vbeta gene families. We detected significant expansions in usage of single Vbeta families in six subjects with DHF and three subjects with DF over the course of infection, but these did not show an association with clinical diagnosis, viral serotype, or HLA alleles. Differences in Vbeta gene usage between subjects with DHF and subjects with DF were of borderline significance. These data suggest that the differences in T cell activation in DHF and DF are quantitative rather than qualitative and that T cells are activated by conventional antigen(s) and not a viral superantigen.

Rights and Permissions

Citation: Am J Trop Med Hyg. 2001 Jan-Feb;64(1-2):41-8.

Related Resources

Link to article in PubMed