UMMS Affiliation

Department of Pediatrics; Department of Medicine

Date

2-1-1989

Document Type

Article

Subjects

*Antibody-Dependent Cell Cytotoxicity; HIV; HIV Antibodies; HIV Antigens; HIV Envelope Protein gp120; HIV Envelope Protein gp41; HIV Seropositivity; Hemophilia A; Humans; Killer Cells, Natural; Retroviridae Proteins; Viral Envelope Proteins

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Antibody-dependent cell-mediated cytotoxicity (ADCC) specific for human immunodeficiency virus (HIV) has been described for HIV-infected individuals. To determine the antigenic specificity of this immune response and to define its relationship to the disease state, an ADCC assay was developed using Epstein-Barr virus-transformed lymphoblastoid cell line targets infected with vaccinia virus vectors expressing HIV proteins. The vaccinia virus vectors induced appropriate HIV proteins (envelope glycoproteins gp160, gp120, and gp41 or gag proteins p55, p40, p24, and p17) in infected lymphoblastoid cell lines as demonstrated by radioimmunoprecipitation and syncytia formation with c8166 cells. Killer cell-mediated, HIV-specific ADCC was found in sera from HIV-seropositive but not HIV-seronegative hemophiliacs. This HIV-specific response was directed against envelope glycoprotein but was completely absent against target cells expressing the HIV gag proteins. The ADCC directed against gp160 was present at serum dilutions up to 1/316,000. There was no correlation between serum ADCC titer and the stage of HIV-related illness as determined by T-helper-cell numbers. These experiments clearly implicated gp160 as the target antigen of HIV-specific ADCC activity following natural infection. Vaccines which stimulate antibodies directed against gp160, which are capable of mediating ADCC against infected cells, could be important for protection against infection by cell-associated virus.

Rights and Permissions

Citation: J Virol. 1989 Feb;63(2):584-90.

Related Resources

Link to Article in PubMed

Journal Title

Journal of virology

PubMed ID

2536094

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