UMMS Affiliation

Department of Medicine, Division of Infectious Diseases and Immunology

Publication Date

6-1-1989

Document Type

Article

Subjects

Animals; Antigens, Viral; Dengue Virus; *Lymphocyte Activation; Male; Mice; Mice, Inbred BALB C; Phenotype; Recombinant Proteins; Species Specificity; T-Lymphocytes; Vero Cells; Viral Proteins

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Definition of the T-lymphocyte responses to dengue viruses should aid in the development of safe and effective vaccines and help to explain the pathophysiology of dengue hemorrhagic fever and dengue shock syndrome. In this study, we demonstrated that dengue virus-specific T lymphocytes were detected in spleen cells from dengue virus-immune mice using an in vitro proliferation assay. Following immunization with a single dose of infectious dengue virus, murine lymphocytes showed increased proliferation when incubated in the presence of viral antigens of the same serotype but not in the presence of control antigens. Depletion experiments with antibody and complement showed that the population of responding cells expressed the Thy1+ L3T4+ Lyt2- phenotype. This indicates that the predominant proliferating cells are T lymphocytes of the helper-inducer phenotype. Dengue virus-specific memory lymphocyte responses were detectable for at least 22 weeks after immunization. The response to primary infection was primarily serotype specific, with some serotype cross-reactivity present at a low level. We demonstrated that lymphocytes from mice immunized with dengue 4 virus proliferate in response to a combination of dengue 4 virus C, pre-M, E, NS1, and NS2a proteins expressed in Sf9 cells with a recombinant baculovirus, and, to a lesser extent, to the dengue 4 virus E protein alone.

Rights and Permissions

Citation: J Virol. 1989 Jun;63(6):2486-91.

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Journal of virology

PubMed ID

2786087

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