Department of Medicine, Division of Infectious Diseases; Center for Infectious Disease and Vaccine Research
Antibodies, Monoclonal; Antigens, CD; Antigens, CD8; Antigens, Differentiation, T-Lymphocyte; Antigens, Viral; Cell Line; Complement System Proteins; Cross Reactions; Cytotoxicity, Immunologic; Dengue Virus; Humans; Lymphocyte Activation; Skin; T-Lymphocytes, Cytotoxic; Ultraviolet Rays
Life Sciences | Medicine and Health Sciences
Stimulation with live dengue virus of peripheral blood mononuclear cells from a dengue virus type 4-immune donor generated virus-specific, serotype-cross-reactive, CD8+, class I-restricted cytotoxic T lymphocytes (CTL) capable of lysing dengue virus-infected cells and cells pulsed with dengue virus antigens of all four serotypes. These CTL lysed autologous fibroblasts infected with vaccinia virus-dengue virus recombinant viruses containing the E gene or several nonstructural dengue virus type 4 genes. These results demonstrate that both dengue virus structural and nonstructural proteins are targets for the cytotoxic T-cell-mediated immune response to dengue virus and suggest that serotype-cross-reactive CD8+ CTL may be important mediators of viral clearance and of virus-induced immunopathology during secondary dengue virus infections.
Rights and Permissions
Citation: J Virol. 1989 Dec;63(12):5086-91.
Journal of virology
Bukowski, Jack F.; Kurane, Ichiro; Lai, Ching-Juh; Bray, Michael; Falgout, Barry; and Ennis, Francis A., "Dengue virus-specific cross-reactive CD8+ human cytotoxic T lymphocytes" (1989). Open Access Articles. 1580.