Department of Pathology
Animals; *Avian leukosis virus; Blotting, Southern; Chickens; DNA Probes; Kidney Neoplasms; Oncogenes; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-fos; Restriction Mapping; Wilms Tumor
Life Sciences | Medicine and Health Sciences
To determine whether c-fos is involved in avian leukosis virus-induced nephroblastoma, 28 tumors from chickens were analyzed for novel fos fragments. DNA from 1 of 16 clonal outgrowths (in chicken 6561) contained novel fos-related EcoRI and KpnI fragments which hybridized to both v-fos and viral probes. Oncogenicity tests using filtered 6561 tumor cell homogenates did not reveal a tumor-inducing transduction of c-fos. We conclude that c-fos is only an occasional target for proviral insertions or new transductions in avian leukosis virus-induced nephroblastoma. The results also identify a polymorphism in c-fos in K28 chickens and demonstrate that unintegrated viral DNA is not a general characteristic of avian leukosis virus-induced nephroblastoma.
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Citation: J Virol. 1990 Jul;64(7):3541-4.
Journal of virology
Collart, K. L.; Aurigemma, Rosemarie E.; Smith, Ralph E.; Kawai, Sadaaki; and Robinson, Harriet L., "Infrequent involvement of c-fos in avian leukosis virus-induced nephroblastoma" (1990). Open Access Articles. 1578.