Department of Medicine, Division of Infectious Diseases and Immunology; Center for Infectious Disease and Vaccine Research
Amino Acid Sequence; CD4-Positive T-Lymphocytes; Clone Cells; Epitopes; HIV Core Protein p24; Humans; Interleukin-2; Interleukin-6; Molecular Sequence Data
Life Sciences | Medicine and Health Sciences
A CD4+ cytotoxic T-lymphocyte (CTL) clone, established from the peripheral blood of a human immunodeficiency virus (HIV)-seropositive donor, lysed autologous target cells that were infected with a recombinant vaccinia virus containing the gag gene of HIV type 1 and target cells pulsed with p24gag construct expressed in Escherichia coli. The recognition of the HLA-DQ-restricted epitope by this clone was further defined by using overlapping synthetic peptides. The epitope recognized by this CD4+ CTL clone (amino acids 140 to 148) overlaps with a CD8+ epitope and is highly conserved among all isolates of HIV type 1 that have been sequenced. Production and secretion of lymphokines such as interleukin-2 and interleukin-6 after specific antigenic stimulation were demonstrated by this gag-specific CD4+ CTL clone.
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Citation: J Virol. 1992 Jan;66(1):608-11.
Journal of virology
Littaua, Rebecca A.; Oldstone, Michael B. A.; Takeda, Akira; and Ennis, Francis A., "A CD4+ cytotoxic T-lymphocyte clone to a conserved epitope on human immunodeficiency virus type 1 p24: cytotoxic activity and secretion of interleukin-2 and interleukin-6" (1992). Open Access Articles. 1568.