UMMS Affiliation

Department of Medicine, Division of Infectious Diseases and Immunology; Center for Infectious Disease and Vaccine Research

Date

4-1-1992

Document Type

Article

Subjects

Antigens, CD4; Cell Division; Cells, Cultured; Chromosome Deletion; Clone Cells; Histocompatibility Antigens Class II; Humans; Phenotype; Smallpox Vaccine; T-Lymphocytes, Cytotoxic; Vaccinia virus

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Vaccinia virus-specific cytotoxic T-lymphocyte (CTL) clones were established from a healthy donor, who had been immunized with vaccinia virus vaccine, by stimulation of peripheral blood lymphocytes with UV-inactivated vaccinia virus antigen. The phenotype of all of the clones established was CD3+ CD4+ CD8- Leu11-. We used a panel of allogenic vaccinia virus-infected B-lymphoblastoid cell lines and demonstrated that some of the clones recognized vaccinia virus epitopes presented by human leukocyte antigen (HLA) class II molecules. Monoclonal antibodies specific for either HLA-DP or HLA-DR determinant reduced the cytotoxicity of specific clones. The HLA-restricted cytotoxicity of the clones is vaccinia virus specific, because vaccinia virus-infected but not influenza virus-infected autologous target cells were lysed. Using vaccinia virus deletion mutants, we found that some of the CTL clones recognize an epitope(s) that lies within the HindIII KF regions of the vaccinia virus genome. These results indicate that heterogeneous CD4+ CTL clones specific for vaccinia virus are induced in response to infection and may be important in recovery from and protection against poxvirus infections.

Rights and Permissions

Citation: J Virol. 1992 Apr;66(4):2274-80.

Related Resources

Link to Article in PubMed

Journal Title

Journal of virology

PubMed ID

1548761

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