UMMS Affiliation

Department of Pathology

Publication Date

4-1-1992

Document Type

Article

Subjects

Antibodies, Monoclonal; Cell Line; HIV Antibodies; HIV Envelope Protein gp120; HIV-1; Humans; Kinetics; Neutralization Tests; Peptide Fragments; Receptors, HIV; T-Lymphocytes; Virus Replication

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The entry of human immunodeficiency virus type 1 into two T-cell lines has been analyzed to determine the relative time courses with which virus entry can be blocked (i) by washing, (ii) by adding a monoclonal antibody to the V3 loop of gp120 that neutralizes without blocking CD4 binding (0.5 beta), or (iii) by adding an antireceptor monoclonal antibody that competes for virus binding (leu3a). During entry into C8166 cells, 50% escape from the wash as well as the anti-V3 loop antibody required 20 min, whereas 50% escape from the leu3a block required 45 minutes. In contrast, during entry into H9 cells, 50% escape from the wash block required 50 min, 50% escape from the anti-V3 loop antibody required 110 min, and 50% escape from the antireceptor antibody required 190 min. These results demonstrate that the times required for entering virus to escape each of the blocks were cell type specific. They also demonstrate that V3 loop-dependent steps occur relatively early in entry and suggest that binding of gp120 to CD4 is important for late as well as early steps in human immunodeficiency virus type 1 entry.

Rights and Permissions

Citation: J Virol. 1992 Apr;66(4):2547-50.

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Journal of virology

PubMed ID

1548779

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