Department of Medicine; Center for Infectious Disease and Vaccine Research
Amino Acid Sequence; Antigens, CD8; Antigens, Differentiation; B-Lymphocytes; Clone Cells; Cytotoxicity, Immunologic; Epitopes; Genetic Vectors; HIV Envelope Protein gp41; HIV-1; HLA-A Antigens; Humans; Molecular Sequence Data; Mutation; Peptide Fragments; Stem Cells; T-Lymphocytes, Cytotoxic; Vaccinia virus
Life Sciences | Medicine and Health Sciences
A human leukocyte antigen A24-restricted CD8+ cytotoxic T-cell clone specific for gp41 of human immunodeficiency virus type 1 was isolated from an infected individual. The epitope was localized to amino acids 584 to 591 (YLKDQQLL, NL43 env sequence) of gp41 by using a panel of recombinant vaccinia viruses that contain truncated env genes and synthetic peptides. The clone killed autologous B-lymphoblastoid cell lines pulsed with a synthetic peptide reflecting the sequence of the IIIB and MN strains. This clone, however, failed to kill target cells pulsed with the peptides that have a mutation from Lys to Arg or Gln at amino acid 585 which is present in some prototype human immunodeficiency virus type 1 strains, e.g., ADA, JFL, SC, ALA1, BAL1, SF2, VRF, SF33, and WMJ2. This finding that a mutation at amino acid 585 on gp41 results in nonrecognition by human leukocyte antigen A24-restricted CD8+ cytotoxic T lymphocytes suggests that antigenic variation at T-cell epitopes contributes to the failure of immune control of human immunodeficiency virus type 1 infections.
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Citation: J Virol. 1992 May;66(5):3151-4.
Journal of virology
Dai, Li Chen; West, Kim; Littaua, Rebecca A.; Takahashi, Kazuo; and Ennis, Francis A., "Mutation of human immunodeficiency virus type 1 at amino acid 585 on gp41 results in loss of killing by CD8+ A24-restricted cytotoxic T lymphocytes" (1992). Open Access Articles. 1564.