Department of Medicine; Center for Infectious Disease and Vaccine Research
Antibodies, Monoclonal; Antigens, CD8; Cells, Cultured; Clone Cells; Cytotoxicity, Immunologic; HLA Antigens; Humans; *Immunity, Cellular; Immunologic Memory; Lymphocyte Activation; Phenotype; T-Lymphocytes, Cytotoxic; Vaccinia virus
Life Sciences | Medicine and Health Sciences
Stimulation of human vaccinia virus immune peripheral blood mononuclear cells in vitro from vaccinia virus-immune donors with live vaccinia virus-infected autologous cells generated vaccinia virus-specific cytotoxic T lymphocytes (CTL) capable of lysing vaccinia virus-infected cells. We generated vaccinia virus-specific CD8+ clones and CD4+ CTL lines by limiting dilution from two donors by using peripheral blood mononuclear cells obtained 2 months or 4 years postrevaccination with vaccinia virus. These results demonstrate that vaccinia virus-specific CTL are generated as a result of immunization of humans with vaccinia virus and that both CD8(+)- and CD4(+)-specific T cells are maintained as memory cells.
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Citation: J Virol. 1993 Mar;67(3):1538-44.
Journal of virology
Demkowicz, Walter E. and Ennis, Francis A., "Vaccinia virus-specific CD8+ cytotoxic T lymphocytes in humans" (1993). Open Access Articles. 1559.