Department of Medicine; Center for Infectious Disease and Vaccine Research
Amino Acid Sequence; Cross Reactions; Cytotoxicity, Immunologic; Dengue Virus; Epitopes; Genotype; HLA-DP Antigens; Humans; Molecular Sequence Data; RNA Helicases; Serine Endopeptidases; Serotyping; T-Lymphocyte Subsets; T-Lymphocytes, Cytotoxic; Viral Nonstructural Proteins
Life Sciences | Medicine and Health Sciences
We previously reported that the clone JK34 was cross-reactive for dengue virus types 1, 2, 3, and 4 and recognized NS3 (I. Kurane, M. A. Brinton, A. L. Samson, and F. A. Ennis, J. Virol. 65:1823-1828, 1991). In the present experiments, we defined the epitope at the amino acid level, with 93 15-mer overlapping peptides which cover the entire NS3. A peptide 4 which contains amino acids 251 to 265 of NS3 sensitized the autologous B lymphoblastoid cell line (LCL) to the lysis by JK34. The smallest peptide recognized by JK34 was a 10-mer peptide which contains amino acids 255 to 264 (EIVDLMCHAT). A monoclonal antibody to HLA-DP inhibited the lysis of epitope peptide-pulsed autologous LCL by JK34. Genotypic typing revealed that the HLA-DP of this donor is DPA1*01, DPB1*0201, which is serologically defined as HLA-DPw2. JK34 lysed peptide 4-pulsed allogeneic LCL which carried HLA-DPw2. These results indicate that HLA-DPw2 is the restriction allele for recognition of this epitope by JK34.
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Citation: J Virol. 1993 Oct;67(10):6285-8.
Journal of virology
Kurane, Ichiro; Dai, Li Chen; Livingston, Peter G.; Reed, Elaine; and Ennis, Francis A., "Definition of an HLA-DPw2-restricted epitope on NS3, recognized by a dengue virus serotype-cross-reactive human CD4+ CD8- cytotoxic T-cell clone" (1993). Open Access Articles. 1558.