Program in Molecular Medicine; Department of Pediatrics
Animals; Base Sequence; Cell Line; Chloramphenicol O-Acetyltransferase; DNA-Binding Proteins; Erythroid-Specific DNA-Binding Factors; Gene Expression Regulation, Viral; HIV Core Protein p24; HIV Long Terminal Repeat; HIV-1; Humans; In Situ Hybridization; Lymphoid Tissue; Molecular Sequence Data; Protein Binding; RNA, Messenger; RNA, Viral; Recombinant Fusion Proteins; Transcription Factors; Transcription, Genetic; Virion; YY1 Transcription Factor
Life Sciences | Medicine and Health Sciences
The transcriptional activity of human immunodeficiency virus type 1 (HIV-1) is affected by many cellular factors. Homologies near the HIV-1 initiator region to the DNA-binding sequences of YY1, a multifunctional transcription factor known to regulate diverse viral and cellular promoters, suggested that YY1 might regulate HIV-1. Antibody to YY1 blocked the formation of complexes by HeLa cell nuclear extract and a DNA oligonucleotide encoding the HIV-1 initiator region. HIV-1 long terminal repeat (LTR) expression, as measured the expression of a transfected LTR-CAT reporter gene, was repressed more than 12-fold by the cotransfection of a YY1 expression vector. HIV-1 production by both COS-1 and CEM cells after transfection of an infectious molecular HIV-1 clone was repressed 7- to 20-fold by cotransfection of a YY1 expression vector. HIV-1 production was also decreased threefold in a CD4-positive lymphocyte cell line chronically infected with HIV-1 (8E5) after transfection of YY1. In situ hybridization studies confirmed that YY1 reduced HIV-1 RNA expression. YY1 may play an important role in the regulation of HIV-1 LTR expression in vivo and virus production by infected cells.
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Citation: J Virol. 1994 Feb;68(2):905-10.
Journal of virology
Margolis, David M.; Somasundaran, Mohan; and Green, Michael R., "Human transcription factor YY1 represses human immunodeficiency virus type 1 transcription and virion production" (1994). Open Access Articles. 1555.