UMMS Affiliation

Department of Medicine; Center for Infectious Disease and Vaccine Research

Date

5-1-1994

Document Type

Article

Subjects

Antigens, CD8; Antigens, Viral; CD4-Positive T-Lymphocytes; Clone Cells; Cross Reactions; Cytotoxicity, Immunologic; Dengue; Dengue Virus; HLA-DR7 Antigen; Humans; Immunization; Serotyping; T-Lymphocytes, Cytotoxic; Viral Envelope Proteins

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

We analyzed dengue virus-specific CD4+ CD8- cytotoxic T lymphocytes (CTL) at the clonal level to further understand their role in dengue virus infections. Stimulation of peripheral blood mononuclear cells from two dengue virus type 4 (D4V)-immune donors with live D4V or noninfectious D4V antigen generated 17 HLA class II-restricted CD4+ CTL capable of specific lysis of dengue virus antigen-treated autologous lymphoblastoid cell lines. Thirteen clones were D4V specific, three clones were cross-reactive for D2V and D4V, and one clone was cross-reactive for D1V, D3V, and D4V. Antigen recognition by six D4V-specific clones and three D2V- and D4V-cross-reactive clones was restricted by HLA-DR7. Five D4V-specific CD4+ CTL clones lysed autologous lymphoblastoid cell lines infected with a dengue virus-vaccinia virus recombinant containing the E gene of D4V, whereas three serotype-cross-reactive CTL clones did not. These results indicate that E-specific clones are serotype specific and HLA-DR7 restricted in these two donors and suggest that a common epitope on E protein may be recognized. E protein-specific CD4+ CTL may be important mediators of virus clearance especially during reinfection with the same serotype as that in primary infection by providing help for virus-specific antibody production and lysis of virus-infected cells.

Rights and Permissions

Citation: J Virol. 1994 May;68(5):3283-8.

Related Resources

Link to Article in PubMed

Journal Title

Journal of virology

PubMed ID

7908702

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