Department of Medicine; Center for Infectious Disease and Vaccine Research
Antigens, CD8; Antigens, Viral; CD4-Positive T-Lymphocytes; Clone Cells; Cross Reactions; Cytotoxicity, Immunologic; Dengue; Dengue Virus; HLA-DR7 Antigen; Humans; Immunization; Serotyping; T-Lymphocytes, Cytotoxic; Viral Envelope Proteins
Life Sciences | Medicine and Health Sciences
We analyzed dengue virus-specific CD4+ CD8- cytotoxic T lymphocytes (CTL) at the clonal level to further understand their role in dengue virus infections. Stimulation of peripheral blood mononuclear cells from two dengue virus type 4 (D4V)-immune donors with live D4V or noninfectious D4V antigen generated 17 HLA class II-restricted CD4+ CTL capable of specific lysis of dengue virus antigen-treated autologous lymphoblastoid cell lines. Thirteen clones were D4V specific, three clones were cross-reactive for D2V and D4V, and one clone was cross-reactive for D1V, D3V, and D4V. Antigen recognition by six D4V-specific clones and three D2V- and D4V-cross-reactive clones was restricted by HLA-DR7. Five D4V-specific CD4+ CTL clones lysed autologous lymphoblastoid cell lines infected with a dengue virus-vaccinia virus recombinant containing the E gene of D4V, whereas three serotype-cross-reactive CTL clones did not. These results indicate that E-specific clones are serotype specific and HLA-DR7 restricted in these two donors and suggest that a common epitope on E protein may be recognized. E protein-specific CD4+ CTL may be important mediators of virus clearance especially during reinfection with the same serotype as that in primary infection by providing help for virus-specific antibody production and lysis of virus-infected cells.
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Citation: J Virol. 1994 May;68(5):3283-8.
Journal of virology
Livingston, Peter G.; Kurane, Ichiro; Lai, Ching-Juh; Bray, Michael; and Ennis, Francis A., "Recognition of envelope protein by dengue virus serotype-specific human CD4+ CD8- cytotoxic T-cell clones" (1994). Open Access Articles. 1554.