UMMS Affiliation

Division of Infectious Diseases and Immunology

Date

10-1-1996

Document Type

Article

Subjects

Animals; Antigens, CD4; Antigens, CD8; CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Dengue Virus; Epitope Mapping; Humans; *Immunophenotyping; Male; Mice; Mice, Inbred BALB C; Sensitivity and Specificity; T-Lymphocyte Subsets

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The target epitopes, serotype specificity, and cytolytic function of dengue virus-specific T cells may influence their theoretical roles in protection against secondary infection as well as the immunopathogenesis of dengue hemorrhagic fever. To study these factors in an experimental system, we isolated dengue virus-specific CD4+ and CD8+ T-cell clones from dengue-2 virus-immunized BALB/c mice. The T-cell response to dengue virus in this mouse strain was heterogeneous; we identified at least five different CD4+ phenotypes and six different CD8+ phenotypes. Individual T-cell clones recognized epitopes on the dengue virus pre-M, E, NSl/NS2A, and NS3 proteins and were restricted by the I-Ad, I-Ed, Ld, and Kd antigens. Both serotype-specific and serotype-cross-reactive clones were isolated in the CD4+ and CD8+ subsets; among CD8+ clones, those that recognized the dengue virus structural proteins were serotype specific whereas those that recognized the nonstructural proteins were serotype cross-reactive. All of the CD8+ and one of five CD4+ clones lysed dengue virus-infected target cells. Using synthetic peptides, we identified an Ld-restricted epitope on the E protein (residues 331 to 339, SPCKIPFEI) and a Kd-restricted epitope on the NS3 protein (residues 296 to 310, ARGYISTRVEM GEAA). These data parallel previous findings of studies using human dengue virus-specific T-cell clones. This experimental mouse system may be useful for studying the role of the virus serotype and HLA haplotype on T-cell responses after primary dengue virus infection.

Rights and Permissions

Citation: J Virol. 1996 Oct;70(10):6540-6.

Related Resources

Link to Article in PubMed

Journal Title

Journal of virology

PubMed ID

8794288

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