Department of Medicine
AIDS Vaccines; Animals; DNA, Recombinant; HIV Antibodies; HIV Envelope Protein gp120; HIV Infections; HIV-1; Humans; Immunization; Neutralization Tests; Rabbits; Vaccines, DNA
Life Sciences | Medicine and Health Sciences
Strategies are needed for human immunodeficiency virus type 1 vaccine development that improves the neutralizing antibody response against primary isolates of the virus. Here we examined recombinant DNA priming followed by subunit protein boosting as a strategy to generate neutralizing antibodies. Both plasmid-based and recombinant protein envelope (Env) glycoprotein immunogens were derived from a primary viral isolate, JR-FL. Serum from rabbits immunized with either gp120 or gp140 DNA vaccines delivered by gene gun inoculation followed by recombinant gp120 protein boosting was capable of neutralizing JR-FL. Neither the DNA vaccines alone nor the gp120 protein alone generated a detectable neutralizing antibody response against this virus. Neutralizing antibody responses using gp120 DNA and gp140 DNA for priming were similar. The results suggest that Env DNA priming followed by gp120 protein boosting provides an advantage over either approach alone for generating a detectable neutralizing antibody response against primary isolates that are not easily neutralized.
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Citation: J Virol. 2005 Jun;79(12):7933-7. Link to article on publisher's site
DOI of Published Version
Journal of virology
Wang, Shixia; Arthos, James; Lawrence, John M.; Van Ryk, Donald I.; Mboudjeka, Innocent; Shen, Siyuan; Chou, Te-hui W.; Montefiori, David C.; and Lu, Shan, "Enhanced immunogenicity of gp120 protein when combined with recombinant DNA priming to generate antibodies that neutralize the JR-FL primary isolate of human immunodeficiency virus type 1" (2005). Open Access Articles. 1518.