UMMS Affiliation

Program in Molecular Medicine

Date

8-17-2005

Document Type

Article

Subjects

Cells, Cultured; Gene Expression Regulation, Viral; HIV Infections; HIV-1; Hela Cells; Humans; Karyopherins; Macrophages; Simian Acquired Immunodeficiency Syndrome; Simian immunodeficiency virus

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

In an in vitro assay employing reconstituted nuclei, importin 7 (IPO7) has been implicated in nuclear translocation of human immunodeficiency virus type 1 (HIV-1) cDNA. Using RNA interference technology, we inhibited expression of IPO7 by 80 to 95% in primary macrophages and in HeLa cells and monitored their ability to support HIV-1 and simian immunodeficiency virus (SIV) cDNA synthesis, nuclear translocation, and infection efficiency. Marked IPO7 deficiency did not alter the rate or extent of HIV-1 or SIV cDNA synthesis or nuclear translocation. The infection efficiency of HIV-1 was similarly unaltered. Therefore, in natural, nondividing targets of HIV-1, IPO7 may be dispensable for infection.

Rights and Permissions

Citation: J Virol. 2005 Sep;79(17):11541-6. Link to article on publisher's site

DOI of Published Version

10.1128/JVI.79.17.11541-11546.2005

Related Resources

Link to Article in PubMed

Journal Title

Journal of virology

PubMed ID

16103209

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