UMMS Affiliation

Department of Molecular Genetics and Microbiology

Publication Date

2-28-2006

Document Type

Article

Subjects

Animals; Cell Line; Chick Embryo; HN Protein; Immunoprecipitation; Membrane Fusion; Newcastle disease virus; Receptors, Cell Surface; Transfection; Viral Fusion Proteins

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Receptor binding of paramyxovirus attachment proteins and the interactions between attachment and fusion (F) proteins are thought to be central to activation of the F protein activity; however, mechanisms involved are unclear. To explore the relationships between Newcastle disease virus (NDV) HN and F protein interactions and HN protein attachment to sialic acid receptors, HN and F protein-containing complexes were detected and quantified by reciprocal coimmunoprecipitation from extracts of transfected avian cells. To inhibit HN protein receptor binding, cells transfected with HN and F protein cDNAs were incubated with neuraminidase from the start of transfection. Under these conditions, no fusion was observed, but amounts of HN and F protein complexes increased twofold over amounts detected in extracts of untreated cells. Stimulation of attachment by incubation of untransfected target cells with neuraminidase-treated HN and F protein-expressing cells resulted in a twofold decrease in amounts of HN and F protein complexes. In contrast, high levels of complexes containing HN protein and an uncleaved F protein (F-K115Q) were detected, and those levels were unaffected by neuraminidase treatment of cell monolayers or by incubation with target cells. These results suggest that HN and F proteins reside in a complex in the absence of receptor binding. Furthermore, the results show that not only receptor binding but also F protein cleavage are necessary for disassociation of the HN and F protein-containing complexes.

Rights and Permissions

Citation: J Virol. 2006 Mar;80(6):2894-903. Link to article on publisher's site

DOI of Published Version

10.1128/JVI.80.6.2894-2903.2006

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Journal of virology

PubMed ID

16501098

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