UMMS Affiliation

Program in Molecular Medicine

Date

7-1-2006

Document Type

Article

Subjects

Adaptor Proteins, Signal Transducing; inhibitors; Cells, Cultured; *Gene Silencing; HIV Infections; HIV Long Terminal Repeat; HIV-1; Humans; Macrophages; Protein Isoforms; Transcription Factors; Virus Integration

Disciplines

Immunology and Infectious Disease | Virology

Abstract

LEDGFp75 is a cellular protein which binds human immunodeficiency virus type 1 (HIV-1) integrase with high specificity and affinity but whose function in infection has not been defined. We infected LEDGFp75-deficient primary macrophages with wild-type HIV in order to assess potential infection phenotypes which would provide clues to LEDGFp75 function. Silencing of LEDGFp75 by 70 to 80% resulted in an average of 53% reduced infection of macrophages by HIV. Analysis of infection intermediates showed that integration, but not two-long-terminal-repeat (2LTR) circles or late cDNAs, was reduced up to 74% in LEDGFp75-deficient macrophages. Therefore, LEDGFp75 has a modest involvement in HIV-1 integration in macrophages.

Comments

Citation: J Virol. 2006 Jul;80(14):7275-80. Link to article on publisher's site

Publisher PDF posted as allowed by the publisher's author rights policy at http://journals.asm.org/site/misc/ASM_Author_Statement.xhtml.

Related Resources

Link to Article in PubMed

Journal Title

Journal of virology

PubMed ID

16809334

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