UMMS Affiliation

Department of Medicine

Date

12-1-2006

Document Type

Article

Subjects

Cell Line, Tumor; DNA-Binding Proteins; Humans; Kinesin; *Mitosis; Oncogene Proteins, Viral; Papillomaviridae; Papillomavirus Infections; Protein Binding; Protein Transport; Viral Proteins; Virus Replication

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

MKlp2 is a kinesin-like motor protein of the central mitotic spindle required for completion of cytokinesis. Papillomavirus E2 is a sequence specific DNA binding protein that regulates viral transcription and replication and is responsible for partitioning viral episomes into daughter cells during cell division. We demonstrate that MKlp2 specifically associates with the E2 protein during mitosis. Using chromatin immunoprecipitation, we show viral genomes are in complex with MKlp2 only within this stage of cell cycle. By immunofluorescence, a subpopulation of papillomavirus E2 colocalizes with MKlp2 in the midbody/midplate during late mitosis. We conclude that during specific stages of mitosis, the papillomavirus E2 protein binds to MKlp2, and infer that association with this motor protein ensures viral genome partitioning during cytokinesis.

Rights and Permissions

Citation: J Virol. 2007 Feb;81(4):1736-45. Epub 2006 Nov 29. Link to article on publisher's site

DOI of Published Version

10.1128/JVI.01638-06

Related Resources

Link to Article in PubMed

Journal Title

Journal of virology

PubMed ID

17135315

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