UMMS Affiliation

Department of Medicine; Department of Cell Biology

Publication Date

12-8-2006

Document Type

Article

Subjects

Animals; Binding Sites; Bovine papillomavirus 1; Cattle; Cell Line; Chromatin Assembly and Disassembly; DNA-Binding Proteins; Hela Cells; Human papillomavirus 16; Humans; Mice; NIH 3T3 Cells; Oncogene Proteins, Viral; Protein Binding; Protein Structure, Tertiary; Trans-Activation (Genetics); Transcription Factors; Viral Proteins

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Papillomavirus E2 is a sequence-specific DNA binding protein that regulates transcription and replication of the viral genome. The transcriptional activities of E2 are typically evaluated by transient transfection of nonreplicating E2-dependent reporters. We sought to address whether E2 activates transcription in an episomal context and its potential interaction with the chromatin remodeling proteins. Using an Epstein-Barr virus-based episomal reporter, we demonstrate that E2 stimulates transcription from an E2-dependent promoter in a chromatin context. This activation is enhanced by the presence of proteins associated with SWI/SNF complexes, which are ATP-dependent chromatin remodeling enzymes. We show that exogenous expression of the Brm ATPase enhances E2 activity in SWI/SNF-deficient cell lines and that the amino-terminal transactivation domain of E2 mediates association with the Brm complex in vivo. Using chromatin immunoprecipitation assays, we demonstrate that Brm enhances promoter occupancy by E2 in an episomal context. Our results demonstrate that E2 activates transcription from an episomal reporter system and reveal a novel property of E2 in collaborating with the Brm chromatin remodeling complex in enhancing transcriptional activation.

Rights and Permissions

Citation: J Virol. 2007 Mar;81(5):2213-20. Epub 2006 Dec 6. Link to article on publisher's site

DOI of Published Version

10.1128/JVI.01746-06

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Journal of virology

PubMed ID

17151122

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.