UMMS Affiliation

Department of Molecular Genetics and Microbiology

Publication Date

3-10-2001

Document Type

Article

Subjects

Alleles; Blotting, Northern; Exoribonucleases; Fungal Proteins; Gene Deletion; Gene Expression Regulation, Fungal; Mutation; Phenotype; Protein Biosynthesis; RNA; RNA Caps; RNA Helicases; RNA, Messenger; *RNA-Binding Proteins; Saccharomyces cerevisiae; *Saccharomyces cerevisiae Proteins; Trans-Activators

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

In Saccharomyces cerevisiae, rapid degradation of nonsense-containing mRNAs requires the decapping enzyme Dcp1p, the 5'-to-3' exoribonuclease Xrn1p, and the three nonsense-mediated mRNA decay (NMD) factors, Upf1p, Nmd2p, and Upf3p. To identify specific functions for the NMD factors, we analyzed the mRNA decay phenotypes of yeast strains containing deletions of DCP1 or XRN1 and UPF1, NMD2, or UPF3. Our results indicate that Upf1p, Nmd2p, and Upf3p regulate decapping and exonucleolytic degradation of nonsense-containing mRNAs. In addition, we show that these factors also regulate the same processes in the degradation of wild-type mRNAs. The participation of the NMD factors in general mRNA degradation suggests that they may regulate an aspect of translation termination common to all transcripts.

Rights and Permissions

Citation: Mol Cell Biol. 2001 Mar;21(5):1515-30. Link to article on publisher's site

DOI of Published Version

10.1128/MCB.21.5.1515-1530.2001

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Molecular and cellular biology

PubMed ID

11238889

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