UMMS Affiliation

Program in Molecular Medicine; Program in Gene Function and Expression; Howard Hughes Medical Institute

Publication Date

10-9-2002

Document Type

Article

Subjects

Acetyltransferases; Chromatin; DNA-Binding Proteins; Dose-Response Relationship, Drug; Formaldehyde; Fungal Proteins; *Gene Expression Regulation, Fungal; Genes, Fungal; Histone Acetyltransferases; Mutation; Polymerase Chain Reaction; Precipitin Tests; *Promoter Regions (Genetics); Protein Binding; Protein Kinases; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; TATA-Box Binding Protein; Temperature; Time Factors; Trans-Activation (Genetics); Trans-Activators; Transcription Factors; *Transcription, Genetic

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The multisubunit Saccharomyces cerevisiae SAGA (Spt-Ada-Gcn5-acetyltransferase) complex is required to activate transcription of a subset of RNA polymerase II-dependent genes. However, the contribution of each SAGA component to transcription activation is relatively unknown. Here, using a formaldehyde-based in vivo cross-linking and chromatin immunoprecipitation assay, we have systematically analyzed the role of SAGA components in the recruitment of TATA-box binding protein (TBP) to SAGA-dependent promoters. We show that recruitment of TBP is diminished at a number of SAGA-dependent promoters in ada1delta, spt7delta, and spt20delta null mutants, consistent with previous biochemical data suggesting that these components maintain the integrity of the SAGA complex. We also find that Spt3p is generally required for TBP binding to SAGA-dependent promoters, consistent with biochemical and genetic experiments, suggesting that Spt3p interacts with and recruits TBP to the core promoter. By contrast, Spt8p, which has been proposed to be required for the interaction between Spt3p and TBP, is required for TBP binding at only a subset of SAGA-dependent promoters. Ada2p and Ada3p are both required for TBP recruitment to Gcn5p-dependent promoters, supporting previous biochemical data that Ada2p and Ada3p are required for the histone acetyltransferase activity of Gcn5p. Finally, our results suggest that TBP-associated-factor components of SAGA are differentially required for TBP binding to SAGA-dependent promoters. In summary, we show that SAGA-dependent promoters require different combinations of SAGA components for TBP recruitment, revealing a complex combinatorial network for transcription activation in vivo.

Rights and Permissions

Citation: Mol Cell Biol. 2002 Nov;22(21):7365-71.

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Molecular and cellular biology

PubMed ID

12370284

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