UMMS Affiliation

Department of Neurobiology; Reppert Lab; Weaver Lab

Date

1-1-2004

Document Type

Article

Subjects

Animals; Binding Sites; Casein Kinases; Cell Cycle Proteins; Circadian Rhythm; Liver; Mice; Mice, Inbred BALB C; Mice, Knockout; Nuclear Proteins; Phosphorylation; Protein Binding; Protein Kinases; Protein Processing, Post-Translational; Protein Structure, Tertiary; Transcription Factors

Disciplines

Neuroscience and Neurobiology

Abstract

The mPER1 and mPER2 proteins have important roles in the circadian clock mechanism, whereas mPER3 is expendable. Here we examine the posttranslational regulation of mPER3 in vivo in mouse liver and compare it to the other mPER proteins to define the salient features required for clock function. Like mPER1 and mPER2, mPER3 is phosphorylated, changes cellular location, and interacts with other clock proteins in a time-dependent manner. Consistent with behavioral data from mPer2/3 and mPer1/3 double-mutant mice, either mPER1 or mPER2 alone can sustain rhythmic posttranslational events. However, mPER3 is unable to sustain molecular rhythmicity in mPer1/2 double-mutant mice. Indeed, mPER3 is always cytoplasmic and is not phosphorylated in the livers of mPer1-deficient mice, suggesting that mPER3 is regulated by mPER1 at a posttranslational level. In vitro studies with chimeric proteins suggest that the inability of mPER3 to support circadian clock function results in part from lack of direct and stable interaction with casein kinase Iepsilon (CKIepsilon). We thus propose that the CKIepsilon-binding domain is critical not only for mPER phosphorylation but also for a functioning circadian clock.

Rights and Permissions

Citation: Mol Cell Biol. 2004 Jan;24(2):584-94.

Comments

Publisher PDF posted as allowed by the publisher's author rights policy at http://journals.asm.org/site/misc/ASM_Author_Statement.xhtml.

Related Resources

Link to Article in PubMed

Journal Title

Molecular and cellular biology

PubMed ID

14701732

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