UMMS Affiliation

Department of Molecular Genetics and Microbiology

Publication Date

6-1-2004

Document Type

Article

Subjects

Amino Acid Sequence; Base Sequence; Binding Sites; DNA, Fungal; Exoribonucleases; Genes, Fungal; Models, Molecular; Molecular Sequence Data; Mutation; Peptide Mapping; Poly(A)-Binding Proteins; Protein Structure, Tertiary; Protein Subunits; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Two-Hybrid System Techniques

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

PAN, a yeast poly(A) nuclease, plays an important nuclear role in the posttranscriptional maturation of mRNA poly(A) tails. The activity of this enzyme is dependent on its Pan2p and Pan3p subunits, as well as the presence of poly(A)-binding protein (Pab1p). We have identified and characterized the associated network of factors controlling the maturation of mRNA poly(A) tails in yeast and defined its relevant protein-protein interactions. Pan3p, a positive regulator of PAN activity, interacts with Pab1p, thus providing substrate specificity for this nuclease. Pab1p also regulates poly(A) tail trimming by interacting with Pbp1p, a factor that appears to negatively regulate PAN. Pan3p and Pbp1p both interact with themselves and with the C terminus of Pab1p. However, the domains required for Pan3p and Pbp1p binding on Pab1p are distinct. Single amino acid changes that disrupt Pan3p interaction with Pab1p have been identified and define a binding pocket in helices 2 and 3 of Pab1p's carboxy terminus. The importance of these amino acids for Pab1p-Pan3p interaction, and poly(A) tail regulation, is underscored by experiments demonstrating that strains harboring substitutions in these residues accumulate mRNAs with long poly(A) tails in vivo.

Rights and Permissions

Citation: Mol Cell Biol. 2004 Jun;24(12):5521-33. Link to article on publisher's site

DOI of Published Version

10.1128/MCB.24.12.5521-5533.2004

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Molecular and cellular biology

PubMed ID

15169912

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