Program in Molecular Medicine
Amino Acid Sequence; Animals; Carrier Proteins; Cell Division; Cell Nucleolus; Cell Nucleus; Cloning, Molecular; Consensus Sequence; Conserved Sequence; Epidermal Growth Factor; Gene Library; Genetic Complementation Test; Hela Cells; Humans; Mice; Molecular Sequence Data; Saccharomyces cerevisiae; *Saccharomyces cerevisiae Proteins; Schizosaccharomyces; Sequence Alignment; Sequence Homology, Amino Acid; Tumor Cells, Cultured; Zinc Fingers
Life Sciences | Medicine and Health Sciences
The zinc finger protein ZPR1 translocates from the cytoplasm to the nucleus after treatment of cells with mitogens. The function of nuclear ZPR1 has not been defined. Here we demonstrate that ZPR1 accumulates in the nucleolus of proliferating cells. The role of ZPR1 was examined using a gene disruption strategy. Cells lacking ZPR1 are not viable. Biochemical analysis demonstrated that the loss of ZPR1 caused disruption of nucleolar function, including preribosomal RNA expression. These data establish ZPR1 as an essential protein that is required for normal nucleolar function in proliferating cells.
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Citation: Mol Biol Cell. 1998 Oct;9(10):2963-71.
Molecular biology of the cell
Galcheva-Gargova, Zoya; Gangwani, Laxman; Konstantinov, Konstantin N.; Mikrut, Monique; Theroux, Steven J.; Enoch, Tamar; and Davis, Roger J., "The cytoplasmic zinc finger protein ZPR1 accumulates in the nucleolus of proliferating cells" (1998). Open Access Articles. 1403.