UMMS Affiliation

Program in Molecular Medicine

Publication Date

10-8-1998

Document Type

Article

Subjects

Amino Acid Sequence; Animals; Carrier Proteins; Cell Division; Cell Nucleolus; Cell Nucleus; Cloning, Molecular; Consensus Sequence; Conserved Sequence; Epidermal Growth Factor; Gene Library; Genetic Complementation Test; Hela Cells; Humans; Mice; Molecular Sequence Data; Saccharomyces cerevisiae; *Saccharomyces cerevisiae Proteins; Schizosaccharomyces; Sequence Alignment; Sequence Homology, Amino Acid; Tumor Cells, Cultured; Zinc Fingers

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The zinc finger protein ZPR1 translocates from the cytoplasm to the nucleus after treatment of cells with mitogens. The function of nuclear ZPR1 has not been defined. Here we demonstrate that ZPR1 accumulates in the nucleolus of proliferating cells. The role of ZPR1 was examined using a gene disruption strategy. Cells lacking ZPR1 are not viable. Biochemical analysis demonstrated that the loss of ZPR1 caused disruption of nucleolar function, including preribosomal RNA expression. These data establish ZPR1 as an essential protein that is required for normal nucleolar function in proliferating cells.

Rights and Permissions

Citation: Mol Biol Cell. 1998 Oct;9(10):2963-71.

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Molecular biology of the cell

PubMed ID

9763455

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