PubMed ID

11907279

UMMS Affiliation

Department of Cell Biology

Date

3-22-2002

Document Type

Article

Subjects

Algal Proteins; Amino Acid Sequence; Animals; Base Sequence; Chlamydomonas; Dynein ATPase; Flagella; Humans; Macromolecular Substances; Models, Molecular; Molecular Sequence Data; Molecular Weight; Peptides; Protein Structure, Secondary; Protein Subunits; Protozoan Proteins; Sequence Alignment

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

To learn more about how dyneins are targeted to specific sites in the flagellum, we have investigated a factor necessary for binding of outer arm dynein to the axonemal microtubules of Chlamydomonas. This factor, termed the outer dynein arm-docking complex (ODA-DC), previously was shown to be missing from axonemes of the outer dynein armless mutants oda1 and oda3. We have now partially purified the ODA-DC, determined that it contains equimolar amounts of M(r) approximately 105,000 and approximately 70,000 proteins plus a third protein of M(r) approximately 25,000, and found that it is associated with the isolated outer arm in a 1:1 molar ratio. We have cloned a full-length cDNA encoding the M(r) approximately 70,000 protein; the sequence predicts a 62.5-kDa protein with potential homologs in higher ciliated organisms, including humans. Sequencing of corresponding cDNA from strain oda1 revealed it has a mutation resulting in a stop codon just downstream of the initiator ATG; thus, it is unable to make the full-length M(r) approximately 70,000 protein. These results demonstrate that the ODA1 gene encodes the M(r) approximately 70,000 protein, and that the protein is essential for assembly of the ODA-DC and the outer dynein arm onto the doublet microtubule.

Rights and Permissions

Citation: Mol Biol Cell. 2002 Mar;13(3):1015-29. Link to article on publisher's site

Related Resources

Link to Article in PubMed

 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.