Title

Compartmentalized phosphorylation of IAP by protein kinase A regulates cytoprotection

UMMS Affiliation

Department of Cancer Biology

Publication Date

7-7-2007

Document Type

Article

Subjects

Animals; Apoptosis; *Cell Compartmentation; Cell Line, Tumor; Cyclic AMP-Dependent Protein Kinases; *Cytoprotection; Humans; Inhibitor of Apoptosis Proteins; Mice; Microtubule-Associated Proteins; Mitochondria; Neoplasms; Phosphorylation; Protein Structure, Secondary; Serine; Transplantation, Heterologous; X-Linked Inhibitor of Apoptosis Protein

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Cell death pathways are likely regulated in specialized subcellular microdomains, but how this occurs is not understood. Here, we show that cyclic AMP-dependent protein kinase A (PKA) phosphorylates the inhibitor of apoptosis (IAP) protein survivin on Ser20 in the cytosol, but not in mitochondria. This phosphorylation event disrupts the binding interface between survivin and its antiapoptotic cofactor, XIAP. Conversely, mitochondrial survivin or a non-PKA phosphorylatable survivin mutant binds XIAP avidly, enhances XIAP stability, synergistically inhibits apoptosis, and accelerates tumor growth, in vivo. Therefore, differential phosphorylation of survivin by PKA in subcellular microdomains regulates tumor cell apoptosis via its interaction with XIAP.

Rights and Permissions

Citation: Mol Cell. 2007 Jul 6;27(1):17-28. Link to article on publisher's site

DOI of Published Version

10.1016/j.molcel.2007.06.004

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Molecular cell

PubMed ID

17612487