ATP stimulates Ca2+ oscillations and contraction in airway smooth muscle cells of mouse lung slices
Department of Physiology
Adenosine Triphosphate; Animals; Calcium; Calcium Signaling; Lung; Male; Mice; Mice, Inbred BALB C; *Muscle Contraction; Muscle, Smooth; Oscillometry
Life Sciences | Medicine and Health Sciences
In airway smooth muscle cells (SMCs) from mouse lung slices, > or =10 microM ATP induced Ca2+ oscillations that were accompanied by airway contraction. After approximately 1 min, the Ca2+ oscillations subsided and the airway relaxed. By contrast, > or =0.5 microM adenosine 5'-O-(3-thiotriphosphate) (nonhydrolyzable) induced Ca2+ oscillations in the SMCs and an associated airway contraction that persisted for >2 min. Adenosine 5'-O-(3-thiotriphosphate)-induced Ca2+ oscillations occurred in the absence of external Ca2+ but were abolished by the phospholipase C inhibitor U-73122 and the inositol 1,4,5-trisphosphate receptor inhibitor xestospongin. Adenosine, AMP, and alpha,beta-methylene ATP had no effect on airway caliber, and the magnitude of the contractile response induced by a variety of nucleotides could be ranked in the following order: ATP = UTP > ADP. These results suggest that the SMC response to ATP is impaired by ATP hydrolysis and mediated via P2Y(2) or P2Y(4) receptors, activating phospholipase C to release Ca2+ via the inositol 1,4,5-trisphosphate receptor. We conclude that ATP can serve as a spasmogen of airway SMCs and that Ca2+ oscillations in SMCs are required to sustain airway contraction.
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Citation: Am J Physiol Lung Cell Mol Physiol. 2002 Dec;283(6):L1271-9. Epub 2002 Aug 2. Link to article on publisher's site
DOI of Published Version
American journal of physiology. Lung cellular and molecular physiology
Bergner, Albrecht and Sanderson, Michael J., "ATP stimulates Ca2+ oscillations and contraction in airway smooth muscle cells of mouse lung slices" (2002). Open Access Articles. 130.