Title

Dam methyltransferase is required for stable lysogeny of the Shiga toxin (Stx2)-encoding bacteriophage 933W of enterohemorrhagic Escherichia coli O157:H7

UMMS Affiliation

Department of Molecular Genetics and Microbiology; Department of Biochemistry and Molecular Pharmacology

Publication Date

11-6-2007

Document Type

Article

Subjects

Coliphages; Enterohemorrhagic Escherichia coli; effects; Kanamycin; Lysogeny; Oligonucleotide Array Sequence Analysis; Polymerase Chain Reaction; Shiga Toxin 2; Site-Specific DNA-Methyltransferase; (Adenine-Specific); Virulence

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Shiga toxin 2 (Stx2), one of the principal virulence factors of enterohemorrhagic Escherichia coli, is encoded by 933W, a lambda-like prophage. 933W prophage induction contributes to Stx2 production, and here, we provide evidence that Dam methyltransferase is essential for maintenance of 933W lysogeny. Our findings are consistent with the idea that the 933W prophage has a relatively low threshold for induction, which may promote Stx2 production during infection.

Rights and Permissions

Citation: J Bacteriol. 2008 Jan;190(1):438-41. Epub 2007 Nov 2. Link to article on publisher's site

DOI of Published Version

10.1128/JB.01373-07

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Journal of bacteriology

PubMed ID

17981979