Dam methyltransferase is required for stable lysogeny of the Shiga toxin (Stx2)-encoding bacteriophage 933W of enterohemorrhagic Escherichia coli O157:H7
Department of Molecular Genetics and Microbiology; Department of Biochemistry and Molecular Pharmacology
Coliphages; Enterohemorrhagic Escherichia coli; effects; Kanamycin; Lysogeny; Oligonucleotide Array Sequence Analysis; Polymerase Chain Reaction; Shiga Toxin 2; Site-Specific DNA-Methyltransferase; (Adenine-Specific); Virulence
Life Sciences | Medicine and Health Sciences
Shiga toxin 2 (Stx2), one of the principal virulence factors of enterohemorrhagic Escherichia coli, is encoded by 933W, a lambda-like prophage. 933W prophage induction contributes to Stx2 production, and here, we provide evidence that Dam methyltransferase is essential for maintenance of 933W lysogeny. Our findings are consistent with the idea that the 933W prophage has a relatively low threshold for induction, which may promote Stx2 production during infection.
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Citation: J Bacteriol. 2008 Jan;190(1):438-41. Epub 2007 Nov 2. Link to article on publisher's site