Transcriptional activation of the histone nuclear factor P (HiNF-P) gene by HiNF-P and its cyclin E/CDK2 responsive co-factor p220NPAT defines a novel autoregulatory loop at the G1/S phase transition
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UMass Chan Affiliations
Department of Cell Biology and Cancer CenterDocument Type
Journal ArticlePublication Date
2007-09-11Keywords
AnimalsCyclin E
Cyclin-Dependent Kinase 2
*G1 Phase
Hela Cells
Homeostasis
Humans
Mice
Models, Biological
Models, Genetic
NIH 3T3 Cells
Promoter Regions (Genetics)
Regulatory Elements, Transcriptional
Repressor Proteins
*S Phase
Signal Transduction
*Trans-Activation (Genetics)
Transcription Factors
Life Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Histone nuclear factor P (HiNF-P) activates histone H4 gene transcription at the G1/S phase transition upon association with its cyclin E/CDK2 responsive co-factor p220NPAT. Here we characterize the gene regulatory pathways that control the proliferation-related expression of HiNF-P. The HiNF-P locus contains a single TATA-less 0.6 kbp promoter with multiple phylogenetically conserved transcription factor recognition motifs. Transient reporter gene assays with HiNF-P promoter deletions show that there are at least three distinct activating regions (-387/-201, -201/-100 and -100/-1) that support maximal transcription. HiNF-P gene transcription is activated by SP1 through the -100/-1 domain and repressed by E2F1 through the -201/-100 domain. The multifunctional co-regulators CBP and p300 also stimulate HiNF-P gene transcription through the -201/-1 core promoter. Importantly, the HiNF-P promoter is activated by both HiNF-P and p220NPAT. This autoregulatory activation is further enhanced by cyclin E and CDK2, while blocked by CDK inhibition (i.e., p57KIP2 p27KIP1, p21CIP). Thus, the HiNF-P gene is a key non-histone target of p220NPAT and HiNF-P. The dependence of HiNF-P gene transcription on cyclin E/CDK2/p220NPAT signaling defines a novel feed-forward loop that may sustain HiNF-P expression in proliferating cells to support the cell cycle regulated synthesis of histone H4 proteins.Source
Gene. 2007 Nov 1;402(1-2):94-102. Epub 2007 Aug 9. Link to article on publisher's site
DOI
10.1016/j.gene.2007.07.027Permanent Link to this Item
http://hdl.handle.net/20.500.14038/38393PubMed ID
17826007Related Resources
ae974a485f413a2113503eed53cd6c53
10.1016/j.gene.2007.07.027