Title

Localization of infection using streptavidin and biotin: an alternative to nonspecific polyclonal immunoglobulin

UMMS Affiliation

Department of Nuclear Medicine

Publication Date

10-1-1992

Document Type

Article

Subjects

Animals; Bacterial Proteins; Biotin; Edetic Acid; Escherichia coli Infections; Immunoglobulin G; Indium Radioisotopes; Lysine; Male; Mice; Pentetic Acid; Radioimmunodetection; Streptavidin; Tissue Distribution

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Since favorable images of infection are obtained with radio-labeled nonspecific IgG, streptavidin has been considered as an alternative protein in this investigation. The advantage of streptavidin is that once localized it may be targeted with radiolabeled biotin. Studies were conducted in a mouse model with an Escherichia coli infection in one thigh. Indium-111-labeled streptavidin showed equivalent localization to the infection as that obtained with 111In-labeled polyclonal nonspecific IgG, however blood levels with streptavidin were lower at all time points; consequently, target-to-blood ratios were improved. Pretargeting with unlabeled streptavidin followed 3 hr later with 111In-labeled biotin showed equivalent localization in the target and reduced activity in all organs sampled. As such, infected thigh-to-normal thigh ratios were improved 3-fold for pretargeting versus either labeled IgG or streptavidin. Improvements in infected thigh-to-liver and blood ratios were greater than 8-fold. Only in the case of kidneys was the ratio unimproved. In conclusion, we have shown that by preadministration of unlabeled streptavidin followed by labeled biotin, infectious lesions in a mouse model may be imaged earlier with lower background levels relative to the administration of labeled nonspecific IgG.

Rights and Permissions

Citation: J Nucl Med. 1992 Oct;33(10):1810-5.

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Journal of nuclear medicine : official publication, Society of Nuclear Medicine

PubMed ID

1403148