UMMS Affiliation

Departments of Psychiatry; Department of Medicine, Division of Endocrinology and Metabolism

Date

11-22-2001

Document Type

Article

Subjects

Age of Onset; Animals; *Behavior, Animal; Calcium; Cell Nucleus; Cerebral Cortex; Corpus Callosum; Corpus Striatum; Dendrites; Disease Models, Animal; Disease Progression; Electrophysiology; Excitatory Amino Acid Agonists; Heterozygote; Huntington Disease; Mice; Mice, Transgenic; Nerve Tissue Proteins; Neurons; Nuclear Proteins; Phenotype; Receptors, N-Methyl-D-Aspartate; Trinucleotide Repeat Expansion

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Neurons in Huntington's disease exhibit selective morphological and subcellular alterations in the striatum and cortex. The link between these neuronal changes and behavioral abnormalities is unclear. We investigated relationships between essential neuronal changes that predict motor impairment and possible involvement of the corticostriatal pathway in developing behavioral phenotypes. We therefore generated heterozygote mice expressing the N-terminal one-third of huntingtin with normal (CT18) or expanded (HD46, HD100) glutamine repeats. The HD mice exhibited motor deficits between 3 and 10 months. The age of onset depended on an expanded polyglutamine length; phenotype severity correlated with increasing age. Neuronal changes in the striatum (nuclear inclusions) preceded the onset of phenotype, whereas cortical changes, especially the accumulation of huntingtin in the nucleus and cytoplasm and the appearance of dysmorphic dendrites, predicted the onset and severity of behavioral deficits. Striatal neurons in the HD mice displayed altered responses to cortical stimulation and to activation by the excitotoxic agent NMDA. Application of NMDA increased intracellular Ca(2+) levels in HD100 neurons compared with wild-type neurons. Results suggest that motor deficits in Huntington's disease arise from cumulative morphological and physiological changes in neurons that impair corticostriatal circuitry.

Rights and Permissions

Citation: J Neurosci. 2001 Dec 1;21(23):9112-23.

Related Resources

Link to Article in PubMed

Journal Title

The Journal of neuroscience : the official journal of the Society for Neuroscience

PubMed ID

11717344

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