Title

Pertussis toxin blocks the inhibitory effects of calcitonin on cyclic AMP accumulation in stimulated cultured human monocytes

UMMS Affiliation

Endocrinology Research Laboratory

Date

11-1-1987

Document Type

Article

Subjects

Adenosine Diphosphate Ribose; Calcitonin; Cell Membrane; Cells, Cultured; Cholera Toxin; Cyclic AMP; Heat; Humans; Monocytes; *Pertussis Toxin; Virulence Factors, Bordetella

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Surface stimulation of fresh or cultured human mononuclear cells by latex particles causes an increase in the accumulation of cyclic AMP that is inhibited by preincubation with calcitonin (CT). Preincubation of cultured monocytes with 500 ng/ml pertussis toxin totally blocks the inhibitory effects of CT at low concentrations of this hormone. The effects of pertussis toxin are dose-related and eliminated by boiling the toxin. Similar preincubations with cholera toxin have no significant effects on subsequent inhibition of surface-stimulated cyclic AMP by CT. Membranes prepared from cultured human monocytes contain a 41,000-dalton protein that is ADP-ribosylated by pertussis toxin and may be the inhibitory guanine nucleotide regulatory protein (Ni) mediating this inhibition.

Rights and Permissions

Citation: J Leukoc Biol. 1987 Nov;42(5):504-9.

Related Resources

Link to Article in PubMed

Journal Title

Journal of leukocyte biology

PubMed ID

2824646