Title

Gene expression in mature neutrophils: early responses to inflammatory stimuli

UMMS Affiliation

Department of Pediatrics; Cancer Center,

Date

11-25-2003

Document Type

Article

Subjects

Chromatin Assembly and Disassembly; Cluster Analysis; Escherichia coli; Gene Expression Profiling; Gene Expression Regulation; Humans; Inflammation Mediators; Lipopolysaccharides; N-Formylmethionine Leucyl-Phenylalanine; Neutrophils; Time Factors; Transcription Factors

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Neutrophils provide an essential defense against bacterial and fungal infection and play a major role in tissue damage during inflammation. Using oligonucleotide microarrays, we have examined the time course of changes in gene expression induced by stimulation with live, opsonized Escherichia coli, soluble lipopolysaccharide, and the chemoattractant formyl-methionyl-leucyl-phenylalanine. The results indicate that activated neutrophils generate a broad and vigorous set of alterations in gene expression. The responses included changes in the levels of transcripts encoding 148 transcription factors and chromatin-remodeling genes and 95 regulators of protein synthesis or stability. Clustering analysis showed distinct temporal patterns with many rapid changes in gene expression within the first hour of exposure. In addition to the temporal clustering of genes, we also observed rather different profiles associated with each stimulus, suggesting that even a nonvirulent organism such as E. coli is able to play a dynamic role in shaping the inflammatory response. Principal component analysis of transcription factor genes demonstrated clear separation of the neutrophil-response clusters from those of resting and stimulated human monocytes. The present study indicates that combinatorial transcriptional regulation including alterations of chromatin structure may play a role in the rapid changes in gene expression that occur in these terminally differentiated cells.

Rights and Permissions

Citation: J Leukoc Biol. 2004 Feb;75(2):358-72. Epub 2003 Nov 21. Link to article on publisher's site

DOI of Published Version

10.1189/jlb.0903412

Related Resources

Link to Article in PubMed

Journal Title

Journal of leukocyte biology

PubMed ID

14634056